Product Name: Ethidium bromide
Synonyms: ETHIDIUM BROMIDE STOCK SOLUTION, 10 mg/ml;OmniPur Ethidium Bromide - CAS 1239-45-8 - Calbiochem;Ethidium bromide Vetec(TM) reagent grade, 95%;ALUMINIUM POTAS.SULPHAT.12H2O;BPW 1X225ML IN 500ML BOTTLE;EZNA KIT SP PLANT DNA MIDI;KIT SP PLANT DNA MIDI EZNA 2 TESTS;PUROMYCIN DIHYDROCHLORIDE ULTRA PURE
CAS: 1239-45-8
MF: C21H20BrN3
MW: 394.31
EINECS: 214-984-6
Product Categories: Purines;Amines;Aromatics;Intermediates &
Fine Chemicals;Pharmaceuticals;marker;Phenanthridine
Melting point 260-262 °C (dec.)(lit.)
density 1.3739 (rough estimate)
refractive index 1.6700 (estimate)
Fp >100°C
storage temp. 2-8°C
solubility H2O: 10 mg/mL, opaque, strongly red
form powder
color Red to dark purple
RIDADR UN 2811 6.1/PG 1
Uses 1. EB can not go through the live cell membrane, but it can dye DNA which goes through the dead cells damaged membrane, it contains a planar perssad which is embeddable stacking basic group, the group fixs position and in close proximity with the basic group, which causing dye bind with DNA and generates fluorescence, because the yield of EB-DNA complexes fluorescence is much greater than the unbound fluorescent dyes, when electrophoresis gel containing free EB, a small amount of DNA can be measured, it is also highly sensitive fluorescent reagent and important fluorescent dye, it is often used to measure DNARNA. When combines with DNA, the replication transcription of DNA can be inhibited.
2. Ethidium bromide is a highly sensitive fluorescent dye for observing DNA of agarose and polyacrylamide gel. Ethidium bromide can emit orange-red signal when it is motivated by standard 302nm ultraviolet light transmittance instrument, Polaroid film or gel image processing system with CCD imaging can be used to shoot. For gel dyes after electrophoresis, storage fluid sample should be diluted to 0.5μg/ml with water, and then gel is incubated for 15-30 minutes. It is usually dispense with bleaching, but if reduces the background color, it can be decolorized in water for 15 minutes. It can then detect DNA bands in UV light box (254nm wavelength).
3. Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10mg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nuc leic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprot ozoal (Trypanosoma).
Uses Ethidium bromide (EtBr) is the most commonly used nucleic acid stain for PAGE or agarose gel electrophoresis. The fluorescence of EtBr increases 21-fold upon binding to double-stranded RNA and 25-fold on binding double-stranded DNA so that destaining the background is not necessary with a low stain concentration (10 μg/ml). Ethidium bromide has been used in a number of fluorimetric assays for nucleic acids. It has been shown to bind to single-stranded DNA (although not as strongly) and triple-stranded DNA. Because of its ability to bind to DNA, EtBr is an inhibitor of DNA polymerase. Antiprotozoal (Trypanosoma).
Uses Intercalating agent and fluorescent label for DNA.
Uses antiprotozoal, intercalcates with DNA
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